General description
Immunoglobulin G (IgG) is the most abundant
immunoglobulin in the human serum. IgM constitutes 5-10% of total
serum immunoglobulins. These immunoglobulins comprise four polypeptide
chains with two heavy (H) chains and two light (L) chains. These polypeptide
chains are inter-linked by disulfide bonds. IgM is a highly conserved
antibody in vertebrates. IgM exists as a pentamer. Both IgG and IgM are
secreted by B cells.
Application
Goat Anti-Mouse IgG & IgM Antibody, HRP conjugate has
been used in western blotting[1](1:400) and immunofluorescence (1:500)[2]
Biochem/physiol Actions
IgG facilitates antibody-dependent cell-mediated
cytotoxicity (ADCC). It also aids in the activation of the classical
pathway of the complement system. IgG neutralizes virus particles and
toxins. IgM, an antibody that is expressed early during an immune response,
aids in engulfing apoptotic cells. It exhibits polyreactive and autoreactive
functionality. IgM induces tissue homeostasis by mediating the clearance of
tissue-based molecules. Lower levels of IgM in serum are associated with increased
autoimmune response and a higher risk of developing infections.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt,
Germany
