biotechrabbit™ MMuLV Reverse Transcriptase is an exceptionally pure DNA polymerase which uses RNA as a substrate and exhibits no measurable proofreading 3'→5' exonuclease function. This enzyme performs cDNA synthesis by extending a DNA primer annealed to an RNA template; it can also make copies from a single-stranded DNA templates.
The enzyme is purified from a recombinant E. coli strain carrying the MMuLV reverse transcriptase gene.
First-strand cDNA synthesis
Generation of labeled cDNA
RNA analysis by primer extension
QPS-101T
THUNDERBIRD® Probe qPCR Mix 1 ml × 1
50x ROX reference dye 50 µl × 1
QPS-101
THUNDERBIRD® Probe qPCR Mix 1.67 ml × 3
50x ROX reference dye 250 µl × 1
QPS-101X5
THUNDERBIRD® Probe qPCR Mix (1.67 ml × 3) x 5
50x ROX reference dye 250 µl × 5
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
Hot Start
Probe
2x Reaction mix
20µl total reaction volume
Store at -20oC
50x ROX reference dye
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