biotechrabbit™ Tris-NTA Amine for high-affinity His-tag binding.
His-tags are one of the most commonly used tags for protein expression analysis. Conventional metal ion chelators, such as nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA), bind His-tags with low affinities in the range of 10 µM. The biotechrabbit Tris-NTA complexes three NTA groups that together bind a 6×His-tag with an affinity that is four orders of magnitude higher (1 nM) than is possible with conventional chelators (10 µM). The binding of His-tags is stoichiometric and single-molecule detection is possible. Binding is reversible: bound His-tags can be released with imidazole or ethylenediaminetetraacetic acid (EDTA).
biotechrabbit™ Tris-NTA is available with a free amine group or conjugated to biotin. It can be used in a large range of applications, including protein detection and labeling, coupling proteins, lipids or cells to surfaces, protein purification and reversible protein modification.
NOTICE TO PURCHASER: LIMITED LICENSE
The use of this product for research purposes is covered by a license from QIAGEN Group. No rights to use this product to perform or offer diagnostic, companion diagnostic, commercial testing or other commercial services for money or money’s worth are granted by the supply of this product expressly, by implication or by estoppel.
Should you wish to use this product for any other purpose not covered by this license, please contact QIAGEN Corporate Business Development at bd@qiagen.com.
