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Sigma-Aldrich® N,N-Dimethylformamide Molecular Biology, ≥99%

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MT-CM-D4551-250ML

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Linear Formula:  HCON(CH3)2

CAS Number:  68-12-2

Molecular Weight:  73.09

Beilstein:  605365

EC Number:  200-679-5

MDL number:  MFCD00003284

UNSPSC Code:  12352111

PubChem Substance ID:  24893883

NACRES:  NA.52

Grade:  Molecular Biology

Assay:  ≥99%

Bp:  153 °C (lit.)

Vapor pressure:  2.7 mmHg ( 20 °C)

Grade:  Molecular Biology

Quality Level:  200

vapor density:  2.5 (vs air)

vapor pressure:  2.7 mmHg ( 20 °C)

Assay:  ≥99%

Form:  liquid

autoignition temp.:  833 °F

expl. lim.:  15.2 %

impurities:  ≤0.5% water

water

refractive index:  n20/D 1.430 (lit.)

bp:  153 °C (lit.)

mp:  −61 °C (lit.)

density:  0.944 g/mL (lit.)

suitability:  suitable for solvent for chromogenic substrates (X-Gal, etc.) used in molecular biology applications.

storage temp.:  room temp

SMILES string:  [H]C(=O)N(C)C

InChI:  1S/C3H7NO/c1-4(2)3-5/h3H,1-2H3

InChI key:  ZMXDDKWLCZADIW-UHFFFAOYSA-N

General description

N, N-Dimethylformamide (DMF) is a solvent with a low evaporation rate, useful for preparing solutions with a variety of hydrophobic organic compounds used in molecular biology applications.

Application

N,N-Dimethylformamide was used to solubilize MTT crystals in cell viability assays.[1] It was also used in feruloyl esterase activity assay in molds exhibiting high activity of the enzyme.[2]

Solvent for many hydrophobic organic compounds.