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Sigma-Aldrich® Fast Blue B Salt Dye content ~95 %, Powder

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Linear Formula:  C14H12N4O2Cl2 · ZnCl2

CAS Number:  14263-94-6

Molecular Weight:  475.47

Colour Index Number:  37235

Beilstein:  1499067

EC Number:  238-153-2

MDL number:  MFCD00075203

UNSPSC Code:  12171500

PubChem Substance ID:  24894113

NACRES:  NA.47

Product Name:  Fast Blue B Salt, Dye content ~95 %

Description:  Technical grade

Quality Level:  200

Form:  powder

Composition:  Dye content, ~95%

technique(s): microbe id | staining: suitable

color:  yellow to dark brown

mp:  >300 °C (lit.)

solubility:  H2O: 1 mg/mL

application(s):

diagnostic assay manufacturing

hematology

histology

storage temp.:  2-8°C

SMILES string:

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2

InChI:

1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4

InChI key:  GPPKNJIWDULNQH-UHFFFAOYSA-J

Application

  • Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.[1]
  • It has been used as a spraying agent in thin layer chromatography for the identification of separated components.[2]
  • It has also been used for the determination of the lipase activity.[3][4]

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye.[5] Similarly, fast blue B is also used for α- and β-glucosidase activity.[6]