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Sigma-Aldrich® Stains-All

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Empirical Formula (Hill Notation):  C30H27BrN2S2

CAS Number:  7423-31-6

Molecular Weight:  559.58

Beilstein:  3865213

EC Number:  231-047-7

MDL number:  MFCD00078788

UNSPSC Code:  12171500

PubChem Substance ID:  24894694

NACRES:  NA.47

Product Name:  Stains-All, ~95%

Assay:  ~95%

Quality Level:  200

Form:  powder

technique(s):  microbe id | staining: suitable

solubility:  chloroform: 40 mg (PLUS 4.0 ml)

application(s):

diagnostic assay manufacturing

hematology

histology

storage temp.:  2-8°C

SMILES string:  [Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC

InChI:

1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1

InChI key:  MPBRYMWMMKKRGC-UHFFFAOYSA-M

Application

Stains-All has been used for the staining of gel made for size determination of hyaluronan.[1][2] It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.[3]

Biochem/physiol Actions

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca2+ binding proteins deep blue to violet, proteins red and lipids yellow-orange.[4] It also stains RNA (bluish purple) and DNA (blue).[5]

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Disclaimer

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.