Usage: sufficient
for 200 colorimetric or fluorometric tests
application(s):
cosmetics
environmental
food and beverages
detection method:
colorimetric
fluorometric
relevant disease(s):
cancer
shipped in: dry
ice
storage temp.: −20°C
General description
Glycerol, also referred to as glycerin or glycerine, is a
3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a
component of phospholipids and triglycerides. Glycerol is a sweet-tasting,
hygroscopic liquid. Glycerol is widely used in food, beverage, and
pharmaceutical formulations[1] and is the main waste by-product of
biodiesel production via transesterification.
Application
Glycerol assay kit has been used to quantify glycerol
release from mouse 3T3-L1 cell culture supernatant samples[2] and to
measure plasma glycerol levels.[3]
This kit is suitable for glycerol detection in foods,
beverages, pharmaceutical formulations, and biological samples.
Biochem/physiol Actions
In this kit, glycerol concentration is determined by a
coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase,
resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm)
product, proportional to the glycerol present. The linear range of detection
for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).
Features and Benefits
Compatible with high-throughput handling systems.
