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Sigma-Aldrich® Nitrotetrazolium Blue chloride

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MT-CM-N6876-250MG

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Linear Formula:  C40H30N10O6 · 2Cl

CAS Number:  298-83-9

Molecular Weight:  817.64

Beilstein:  4115923

MDL number:  MFCD00012159

UNSPSC Code:  12171500

PubChem Substance ID:  24897795

NACRES:  NA.47

Quality Level:  200

Assay:  ≥90.0% (HPLC)

Form:  powder

Impurities:  ≤10% solvent

Solubility:  H2O: 10 mg/mL

application(s):

diagnostic assay manufacturing

hematology

histology

storage temp.:  2-8°C

SMILES string:

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI:

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key:  FSVCQIDHPKZJSO-UHFFFAOYSA-L

General description

Substrate for dehydrogenases[1] and other oxidases.[2]

Application

  • Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.[3]
  • It has been used as a substrate for western blot detection.[4]
  • It has been used for the staining of histological tissue sections.[5]

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.[6][7]

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis