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Merck 4-Nitrophenyl butyrate

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MT-CM-N9876

Synonym(s): Blue-Dyed Latex Beads, Deep Blue Latex Beads 


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Empirical Formula (Hill Notation):  C10H11NO4

CAS Number:  2635-84-9

Molecular Weight:  209.20

Beilstein:  1879729

MDL number:  MFCD00047734

UNSPSC Code:  12352204

PubChem Substance ID:  24897915

NACRES:  NA.83

Product Name:  4-Nitrophenyl butyrate, ≥98%

Assay:  ≥98%

Form:  liquid

Density:  1.19 g/mL at 20 °C (lit.)

storage temp.:  −20°C

SMILES string:  CCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI:  1S/C10H11NO4/c1-2-3-10(12)15-9-6-4-8(5-7-9)11(13)14/h4-7H,2-3H2,1H3

InChI key:  DVDUMIQZEUTAGK-UHFFFAOYSA-N

General description

4-Nitrophenyl butyrate is a substrate for esterase,[1] and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.[2]

Application

4-nitrophenyl butyrate has been used:


  • as a substrate for esterase activity in monocyte-derived macrophage cell line in response to polyethylene[3]
  • as a substrate for mycobacterial phospholipase A and mycobacterial cutinase activity[4]
  • as a substrate to determine the effect of buffers and solvents on human carboxylesterase[5]