General description
Protein G is an immunoglobulin (IgG)-binding bacterial cell
wall protein isolated from group G streptococcal strain, G-148.[1] This
protein can be extracted from the cells by papain digestion and purified by the
sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity
chromatography on Sepharose-coupled human IgG, and gel chromatography on
Sephadex G-200.[2] The binding between protein G and various polyclonal
and monoclonal IgG is basically pH dependent between 2.8 and 10, with the
strongest binding at pH 4 and 5, and weakest at pH 10.[3] It acts as a
powerful reagent for the detection of IgG.[1]
P3296-5Ml′s updated product number is GE17-0618-01
Application
Protein G-Sepharose™ is used in affinity
chromatography, protein chromatography, antibody purification and
characterization, immunoaffinity matrices, protein A, G and L resins, protein
interaction, and purification and detection. Protein G-Sepharose™ has
been used to develop a strategy to confirm the presence of anti-erythropoietin
neutralizing antibodies in human serum as well as to compare methods for
depletion of albumin and IgG from equine serum.
Physical form
Suspension in 20% ethanol
Preparation Note
Prepared with recombinant streptococcal Protein G from which
the albumin-binding region has been genetically deleted
Legal Information
Sepharose is a trademark of Cytiva
