General description
A tris-equilibrated phenol solution is a clear liquid capped
with a layer of Tris buffer. Phenol solutions can be used for a wide variety of
molecular biology applications. Organic compounds like phenols have an aromatic
carbocyclic ring with a hydroxyl group attached directly to it. The common name
for monohydroxybenzene is phenol.[1]
Application
Phenol solution has been used:
- as a
component of the digestion reaction mix to prepare RNase-free plasmids
for in vitro translation in a rabbit reticulocyte lysate
- for
extraction of DNA from Arabidopsis thaliana Rosette leaves
- in
the extraction of DNA from blood on preservation paper by standard
phenol-chloroform protocol
- as a
component of extraction buffer to extract RNA from fruit pulp
- for
the extraction of protein from roots[2]
- for
the isolation of fecal DNA[3]
- for
the extraction of caged RNA[4]
Biochem/physiol Actions
The phenol-chloroform extraction method is widely employed
to extract nucleic acids. Even though phenol does not completely inhibit RNase
activity, its unique characteristic makes it beneficial for the isolation of
nucleic acids when used in combination with chloroform and alcohol.[5]
Other Notes
- 1
bottle Tris-equilabrated Phenol (product number P4557)
- Equilibration
Buffer (product B5658)
