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Sigma-Aldrich® TWEEN® 80

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MT-CM-P4780-100ML

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CAS Number:  9005-65-6

MDL number:  MFCD00082107

UNSPSC Code:  12161700

NACRES:  NA.25

Description:  non-ionic

Quality Level:  200

Form:  viscous liquid

mol wt:  average mol wt ~1310

composition:  Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

technique(s):

cell culture | insect: suitable

cell culture | mammalian: suitable

CMC:  0.012 mM

Density:

1.06 g/mL at 25 °C (lit.)

1.07 g/cm3 at 25 °C (lit.)

application(s):  (ELISA and immunoassays)

SMILES string:

CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

InChI:

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChI key:  RGPBUVUVZKQNHD-MDZDMXLPSA-N

General description

TWEEN 80 is a polyethylene sorbitol ester[1] and has a molecular weight of 1.31 kDa. It is useful as a stabilizer and emulsifier commercially. It is known to be used in ice-cream for its drying property.[2]

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.

TWEEN® 80 has been used:


  • in Listeria monocytogenes enumeration using filtration method[3]
  • as a hydrophilic emulsifier in the preparation of W1/O/W2 emulsion[4]
  • as a component of 7H9 plus oleic albumin dextrose catalase (OADC) in Mycobacterium Bovis bacillus Calmette–Guerin (BCG) Pasteur strain culturing[5]

 

Legal Information

TWEEN is a registered trademark of Croda International PLC