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Sigma-Aldrich® Ponceau S solution BioReagent, 0.1 % (w/v) in 5% acetic acid

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Empirical Formula (Hill Notation):  C22H12N4Na4O13S4

CAS Number:  6226-79-5

Molecular Weight:  760.57

MDL number:  MFCD00003892

UNSPSC Code:  12171500

PubChem Substance ID:  24898827

NACRES:  NA.47

Product Name:

Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

Description:  for protein analysis

Quality Level:  200

product line:  BioReagent

form:  liquid

concentration:  0.1 % (w/v) in 5% acetic acid

technique(s):  western blot: suitable

density:  1.01 g/cm3 at 20 °C

suitability:

suitable (for use in cellulose acetate electrophoresis)

suitable for electrophoresis

application(s):

diagnostic assay manufacturing

hematology

histology

storage temp.:  room temp

SMILES string:

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI:

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

InChI key:  KQHKSGRIBYJYFX-WNDMICSESA-J

General description

Ponceau S is the sodium salt of a diazo dye.[1] Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting).[1] A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Application

Ponceau S solution has been used for membrane staining (Western blotting).[2][3][4]