For unbiased lysis of microbes and/or solid tissue in
TRIzol, use the ZR BashingBead Lysis Tubes (select option) for efficient
homogenization of hard-to-lyse samples prior to RNA purification with the Direct-zol RNA kit.
The Direct-zol RNA kit isolates total RNA (including small/miRNAs)
from any sample in TRIzol in just 7 minutes.
ZR BashingBead Lysis Tubes (S6003 for
animal/plant tissue, S6012 for
microbes, S6014 for
microbes in tissue/insects) are 2.0 ml screwcap tubes with high density,
fracture resistant, chemically inert, ceramic beads and is used upstream for
the disruption of hard-to-lyse samples. Simply collect your sample in
TRIzol, transfer the lysate into a Lysis Tube and perform mechanical
homogenization (high-speed bench homogenizer adapted for 2.0 ml
tubes). Then process the TRIzol lysate with the Direct-zol RNA kit by
binding directly to the spin-column, wash and elute. DNA-free, RNA is
ready to use for any downstream application such as NGS, RT/qPCR and etc.
