General description
RNAzol RT is a quick and convenient reagent for use in the
single-step isolation of total and small RNA from biological samples of human,
animal, plant, yeast, bacterial, and viral origin.[1][2][3] A convenient
single-step liquid phase separation results in the isolation of RNA from DNA,
protein, polysaccharides, and other molecules. RNAzol® RT can
be used to isolate separate fractions of mRNA and micro RNA or to isolate total
RNA, containing all classes of RNA in a single fraction.
This product, a mixture of guanidine thiocyanate and phenol in a monophase
solution, effectively dissolves DNA, RNA, and protein on homogenization or
lysis of tissue sample. Addition of water to the mixture allows for the
precipitation of DNA, proteins, polysaccharides and other molecules, which can
then be removed by centrifugation. RNA can then be isolated by alcohol
precipitation, washing and solubilization. Chloroform-induced phase separation
is not necessary. One mL of RNAzol® RT is sufficient to isolate
RNA from up to 100 mg of tissue or 10e7 cells.
This is one of the most effective methods for isolating total and small RNA and
can be completed at room temperature in less than 1 hour starting with fresh
tissue or cells. The procedure is very effective for isolating RNA molecules of
all types: large nuclear RNA, rRNA, mRNA, small RNA and micro RNA. The
resulting RNA is intact with little or no contaminating DNA and protein.
Application
Isolated RNA can be used for Northern blots, RNase
protection assay, microarrays, PCR, and other molecular biology applications.
RNAzol RT is a highly effective reagent for the single-step
isolation of total and small RNA from human, animal, plant, bacterial and viral
origin samples. The RNA isolation method based on this reagent is performed at
room temperature, without the use of chloroform for phase separation, and
allows for use in RT-PCR without requiring DNase treatment.
Features and Benefits
- Isolates
micro RNA and total RNA isolation of pure and undegraded RNA from
biological samples
- High
yields and increased quality of isolated RNA
- Completed
at room temperature in less than an hour starting with fresh tissue or
cells
Other Notes
2024
CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Legal Information
RNAZol is a registered trademark of Molecular Research
Center, Inc.
