General description
RPMI 1640 Medium was developed at Roswell Park Memorial
Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A
Medium, it was formulated to support lymphoblastoid cells in suspension
culture, but it has since been shown to support a wide variety of cells that
are anchorage-dependent. Originally intended to be used with a serum
supplement, RPMI 1640 has been shown to support several cell lines in the
absence of serum. It has also been widely used in fusion protocols and in the
growth of hybrid cells. This medium is suitable for culturing human normal and
neoplastic leukocytes.
Roswell Park Memorial Institute medium is commonly known as
RPMI. It is a form of medium used in cell culture and tissue culture. This
medium contains phosphate and is formulated for use in a 5% carbon dioxide
atmosphere. It is used for the growth of human lymphoid cells.
Application
RPMI-1640 Medium is used for the following applications:
- used
in cell culture medium (i.e. Murine thymoma EL-4 cells were cultured in
RPMI 1640 medium supplemented with other components)[1]
- used
for the mycobacterial infection of splenocytes (in antibiotic-free RPMI
1640 medium)[2]
- used
for cell isolation and culture[3][4]
- used
in culture medium (as one of the component) during lymphocyte separation
and culture, ELISPOT Assay, Ex Vivo proliferation
assay[5]
- used
in medium for gp39 gene expression and CD40-Immunoglobuin binding
assays[6]
- used
for the preparation of antifungal agents[7][8]
Preparation Note
Formulated to contain 10.4 grams of powder per liter of
medium.
Supplement with 2.0 g/L sodium bicarbonate.
