BlueZol is a ready-to-use reagent for the isolation of total RNA from various biological materials such as animal and plant tissues, cell culture and bacterial cells.
Homogenisation of a biological sample in BlueZol leads to a separation into three phases: an aqueous upper phase, an organic lower phase and an interphase. The RNA remains in the aqueous phase and its purification is followed by precipitation in isopropyl alcohol. The highly effective RNase inhibitory property of Bluezol protects the integrity of the RNA during lysis and results in the isolation of high-quality RNA. The purified RNA is ideal for any downstream applications such as RT-PCR, in vitro translation Northern Blotting, RNase protection assays or dot blot hybridization.
BlueZol can be used for the simultaneous isolation of RNA, DNA and protein from one sample. The DNA remains in the interphase and the proteins in the organic phase. After purification DNA can be used for PCR and Southern Blotting and the proteins for Western Blotting.
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Muta.2 • GGVSE/ADR: 6.1 III UN2810 • IATA: 6.1 III UN2810 • WGK: 2 • HS: 38229000
Storage Temperature: +2 °C to +8 °C