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Merck Trypan Blue solution

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MT-CM-T8154.0100

0.4%, liquid, sterile-filtered, suitable for cell culture 100mL.

Synonym(s):  Blue cell viability dye

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1,750.00 ฿ 1750.0 THB 1,750.00 ฿

1,750.00 ฿

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Empirical Formula (Hill Notation):  C34H24N6Na4O14S4

CAS Number:  72-57-1

Molecular Weight:  960.81

Beilstein:  4360496

MDL number:  MFCD00003969

UNSPSC Code:  12352207

PubChem Substance ID:  57654704

NACRES:  NA.75

Sterility:  sterile-filtered

Quality Level:  200

Form:  liquid

storage condition:  dry at room temperature

concentration:  0.4%

technique(s):

cell culture | mammalian: suitable

tissue processing: suitable

application(s):  cell analysis

shipped in:  ambient

SMILES string:

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI:

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4



General description

For the same product in a convenient lyophilized (powder) format, please see T6146

Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue.[1] It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.[2]

Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane.[3] This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.

Application

Trypan Blue solution has been used:


  • in cell viability assay to count viable and dead cells[4]
  • in rescue assay to count viable cells[4]
  • in trypan blue exclusion counting method to determine proliferation curve[5]
  • as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion[6]
  • in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability[7]
  • to count GEC cells using a hemocytometer[7]

 

Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.