(X-Gal)
C14H15BrClNO6 • Mr 408.6 • CAS [7240-90-6]
X-Gal is a well known histochemical substrate used to detect
the β-galactosidase enzyme (1, 2, 3, 4, 9, 10). Identification of lacf-colonies
(8). X-gal is used to distinguish recombinant plasmids from parental vectors in
cloning experiments using vectors containing the lacZ or lacZ [DMvP1](-)peptide
gene (2, 3, 5). Upon hydrolysis, X-Gal yields a localized, insoluble blue
precipitate making it exceptionally useful in blotting, immunocytochemical, and
ELISA assays. X-Gal has been used for the detection of coliforms (E. coli)
in municipal water supplies (6) and food products (7). X-Gal is often used in
conjunction with IPTG (Isopropyl-β-D-thiogalactopyranoside, cat. no. 26600)
which binds and inhibits the lac repressor thus inducing β-galactosidase
activity.
Stock solution: 20 mg/ml in DMF. Store in aliquots at -20 °C in the
dark. For detection of transformants, use in final concentration of 40 µg/ml.
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Purity (HPLC)
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min. 99.0 %
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