-
  1. All Products
  2. Microbiology
  3. Promega® T4 DNA Ligase

Promega® T4 DNA Ligase

https://mtmedicalscience.com/web/image/product.template/2289/image_1920?unique=44b9b5c
(0 review)

MT-MB-M1801



*กรณีแสดงราคา 0 บาท กรุณาติดต่อบริษัทเพื่อสอบถาม ราคาขาย*

0.00 ฿ 0.0 THB 0.00 ฿

0.00 ฿

Not Available For Sale

This combination does not exist.

Contact Us

Joins Two DNA Strands with Cohesive or Blunt Ends

  • Use with 5´, 3´ or blunt-ended dsDNA (e.g., inserts and vectors)
  • Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications
  • Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule
  • Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated

T4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The enzyme will not join single-stranded nucleic acids. T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP.

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Reference

  1. Engler, M.J. and Richardson, C.C. (1982) In: The Enzymes, Boyer, P.D., ed., Academic Press, New York, NY.

Note: Units listed are Weiss units.

Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.

Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.

https://worldwide.promega.com/-/media/files/resources/protocols/product-information-sheets/g/t4-dna-ligase-blue-white-cloning-qualified-protocol.pdf?rev=e4c228c69dba4d0e92f5b1fd2599c663

General description

D-(+)-Glucose is a monosaccharide which is the main source of energy in the form of ATP for living organisms.[1] The effect of adding glucose as a supplement to the infection media of Cryptosporidium parvum oocysts has been studied.[2] The polymer-bound glucose template has been demonstrated to be useful in the multi-step parallel synthesis of various compounds.[3]

Application

D-(+)-Glucose has been used as a standard for the estimation of total sugar in hydrolyzed starch by phenol-sulfuric acid method.[4]

It has been used in the preparation of the following liquid media for culturing yeast cells:[5]


  • CM glucose broth minus HIS (histidine)
  • CM glucose broth minus URA (uracil)
  • CM glucose broth minus TRP (tryptophan)

Preparation Note

The material may produce a yellowish solution if prepared at a higher concentration than 2 g + 15mL.

Other Notes

To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.