T4 Polynucleotide Kinase catalyzes the transfer of the
γ-phosphate from ATP to the 5´-terminus of polynucleotides or to
mononucleotides bearing a 5´-hydroxyl group. The enzyme may be used to
phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent
manipulations such as ligation and cloning. T4 PNK is provided with 10X
Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM
DTT.
Choose Your Configuration: Learn more about our
custom options for this product at: www.promega.com/custom/
Storage Buffer: 20mM Tris-HCl (pH 7.5), 25mM
KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP and 50% (v/v) glycerol.
Source: Recombinant E. coli strain.
QC Tests: Activity, DNase, RNase, endonuclease,
end-labeling, blue/white cloning assay.
Unit Definition: One unit is defined as the
amount of enzyme required to catalyze the transfer of 1nmol of phosphate to the
5´-OH end of a polynucleotide from [γ-32P]ATP in 30 minutes at 37°C.
The reaction conditions are: 40mM Tris-HCl (pH 7.5 at 25°C), 10mM MgCl2,
5mM DTT, 0.1mM [γ-32P] ATP and 0.5mM 5´-OH polynucleotide end
concentration.
https://worldwide.promega.com/-/media/files/resources/protocols/technical-bulletins/101/t4-polynucleotide-kinase-protocol.pdf?rev=8b224ee9a08942b5a366c1bc9e8639b7