AccuStart II GelTrack® PCR SuperMix is a 2X
concentrated, ready-to-use reaction cocktail for routine PCR amplification of
DNA fragments up to 4 kb. It contains all components, except primers and
template. This kit improves assay reproducibility, and reduces the risk of contamination.
A key component is AccuStart II Taq DNA polymerase which contains ultra-pure
monoclonal antibodies that arrest polymerase prior to the initial PCR
denaturation step. Hot start antibodies are rapidly and irreversibly denatured
(1 minute at 94ºC), releasing a high yielding Taq DNA polymerase mutant with
absolute maximum processivity and velocity from the first cycle. This enables
specific and efficient primer extension with the convenience of room
temperature reaction assembly.
GelTrack Loading Dye is a mixture of blue and yellow
electrophoresis-tracking dyes that migrate at approximately 4kb and 50 bp, and
comes pre-mixed with the PCR reagents.
