AccuStart II PCR ToughMix is a 2X concentrated ready-to-use
reaction cocktail for PCR amplification of DNA templates that overcomes many
known inhibitors of PCR often present in crude samples extracted from
environmental specimens, plant tissues, or animal tissues. The only user
supplied components are DNA template and molecular grade water.
A key component to AccuStart PCR ToughMix is an ultra pure,
highly processive thermostable DNA polymerase that is combined with high
avidity monoclonal antibodies. These antibodies bind the polymerase and keep it
inactive prior to the initial PCR denaturation step. This enables specific and
efficient primer extension with the convenience of room temperature reaction
assembly. Similar to Taq DNA polymerase, the activated polymerase in AccuStart
II PCR ToughMix possesses 5'>3' DNA polymerase activity and a double-strand
specific 5'>3' exonuclease. The polymerase does not have 3'-exonuclease
activity and is free of any contaminating endo or exonuclease activities. PCR
products generally contain non-templated dA additions and can be cloned using
vectors that have a single 3'-overhanging thymine residue on each end.
GelTrack Loading Dye is a mixture of blue and yellow
electrophoresis-tracking dyes that migrate at approximately 4kb and 50 bp. This
optional component simplifies post-PCR analysis with gel electrophoresis and
eliminates potential for cross contamination by enabling direct transfer
of PCR products to the gel sample wells.
The GelTrack Loading Dye solution is not included with the sample size
95142-100.
