General description
Goat polyclonal anti-Mouse IgG (whole molecule)−Alkaline
Phosphatase antibody is reactive with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and
IgM. Cross reactivity of the antibody conjugate is determined by ELISA. The
conjugate shows no reactivity with rat IgG or rat serum proteins.
Immunoglobulins (Igs) belong to the immunoglobulin
super-family. IgG is an abundant immunoglobulin present in human serum. The
four classes of IgG are IgG1, IgG2, IgG3, and IgG4.
Mouse IgG is a plasma B cell derived antibody isotype
defined by its heavy chain. IgG is the most abundant antibody isotype found in
mouse serum. IgG crosses the placental barrier, is a complement activator and
binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with
the status of disease or infection.
Application
Anti-Mouse IgG (whole molecule) Alkaline Phosphatase
antibody produced in goat has been used as a secondary antibody in:
- histological
studies in heart and liver grafts
- immunohistochemical
studies of gangliosides and immunostaining of lymphoid organs
- western
blot detection in blood samples
Surfactant Protein A was detected in bronchoalveolar fluid
using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at
μg/ml in TBS/Tween containing final concentration of 0.5M NaCl.
The level of antibodies specific to P. aeuginosa present
in immunized mouse sera was determined by ELISA using alkaline
phosphatase-conjugated goat anti-mouse IgG as the secondary antibody at a
dilution of 1:1000.
Biochem/physiol Actions
IgG1 deficiency results in hypogammaglobulinemia. IgG2
deficiency increases susceptibility to bacterial infections. IgG3 mediates
effector functions and IgG4 is associated with asymptomatic infection.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine,
1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium
azide.
Preparation Note
Adsorbed to reduce background with rat samples.
Other Notes
Antibody adsorbed with rat serum proteins.
Disclaimer
Unless otherwise stated in our catalog or other company
documentation accompanying the product(s), our products are intended for
research use only and are not to be used for any other purpose, which includes
but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,
ex vivo or in vivo therapeutic uses or any type of consumption or application
to humans or animals.
