abcam Calcium Assay Kit (Colorimetric)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.2 – 1 mM).

How the assay works

In the calcium assay protocol, a chromogenic complex is formed between calcium ions and 0-cresolphthalein. The complex is measured at OD = 575 nm.

Calcium assay protocol summary

• - Add samples and standards to wells
• - Add chromogenic reagent, add calcium assay buffer and incubate for 5-10 min at room temp
• - Analyze with microplate reader

Related Calcium assay products

Calcium assay kit ab102505 is designed for use with liquid samples such as biological fluids, cell lysates, and tissue extracts.

For the analysis of calcium flux in cell cultures in imaging assays, we supply Fluo-8 Calcium Flux Assay Kits. No wash kit Fluo-8 Calcium Flux Assay Kit Fluo-8 Calcium Flux Assay Kit - No Washab112129 is generally preferred as the workflow is simpler. The alternative is Medium removal kit Fluo-8 Calcium Flux Assay Kit Fluo-8 Calcium Assay Kit - Medium Removalab112128. Note - calcium flus assays are also referred to as calcium influx assays.

For the analysis of calcium flux in cultured cells in a flow cytometer, we supply Calcium Flux Assay Kit (Flow cytometry) (Calcium Flux Assay Kit (Flow cytometry)ab233472).

For the imaging of calcium deposits in paraffin sections, we supply Von Kossa (Calcium Stain) Kit Von Kossa Stain Kit (Calcium Stain)ab150687.

Getting the best performance from ab102505

This calcium assay detects free calcium ions only.

Other notes

This product is manufactured by BioVision, an Abcam company and was previously called K380 Calcium Colorimetric Assay Kit. K380-250 is the same size as the 250 test size of ab102505.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.

It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye