How the assay works
In the amylase assay protocol, alpha-Amylase will cleave the
substrate ethylidene-pNP-G7 to produce smaller fragments that are eventually
modified by alpha-glucosidase. This causes the release of a chromophore that
can then be measured at OD = 405 nm.
The assay can detect α-amylase content as low as 0.2 mU.
Amylase assay protocol summary:
- Add samples and standards to wells
- Prepare standard curve
- Add reaction mix
- Measure optical density (OD405 nm) in a kinetic mode at 25°C every 2-3 min
for 30-60 min on a microplate reader
How other researchers are using
Amylase Assay Kit (Colorimetric) has been used in a variety
of sample type including:
Mouse blood serum 1
Mouse pancreas samples 2
Human pancreatic cancer cell lines 3
References:
1-Thompson D et al. 2023
2-Thompson D et al. 2023
3-Xue Q et al. 2023
Related and recommended products
To screen potential inhibitors of Alpha-Amylase we
recommend:
- Alpha-Amylase Inhibitor Screening Kit (Colorimetric) Alpha-Amylase
Inhibitor Screening Kit (Colorimetric)ab283391
This product is manufactured by BioVision, an Abcam company
and was previously called K711 Amylase Activity Colorimetric Assay Kit.
K711-100 is the same size as the 100 test size of ab102523.
Amylases are enzymes that break starch down to sugar
molecules. α-amylase is the major form of amylase found in humans and other
mammals as well as an enzyme present in seeds, or in fungi (baker's yeast for
instance). α-amylase is a calcium metalloenzyme, completely unable to function
in the absence of calcium. In human physiology, both the salivary and
pancreatic amylases are major digestive enzymes.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
