Detection method
Colorimetric
Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant,
Serum, Other biological fluids
Assay type
Enzyme activity
Assay time
1h 30m
Assay Platform
Microplate reader
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Lipase Assay Kit II (Colorimetric) (ab102525) provides a simple, sensitive, and reliable assay for rapid analysis of Lipase activity.
In the lipase assay, Lipases hydrolyze a specific substrate to generate a product which reacts with the DTNB probe to generate color (Absorbance=412 nm). This kit is suitable for high throughput analysis (HTP).
Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader at 3 min and at between 60 and 90 min
This product is manufactured by BioVision, an Abcam company and was previously called K723 Lipase Activity Colorimetric Assay Kit II. K723-100 is the same size as the 100 test size of ab102525.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
