How the assay works
In the trypsin activity assay protocol, trypsin cleaves a
substrate to generate p-nitroaniline (p-NA) which is detected at OD = 405 nm.
Since the color intensity is proportional to p-NA content, trypsin activity can
be accurately measured.
Trypsin Activity Assay Kit (Colorimetric) protocol
summary :
- Add samples, positive control and standards to wells
- Add chymotrypsin inhibitor to sample and positive control wells and incubate
for 10 min at room temp
- Add reaction mix and incubate 25°C protected from light
- Measure optical density (OD 405nm) in a kinetic mode at 25°C for 1 – 2 hours
protected from light
How other researchers are using
Trypsin Activity Assay Kit (Colorimetric) has been used in a
variety of sample type including:
Mouse intestinal tissues 1
Mouse Cecal tissue 2
Mouse Pancreatic Acinar Cells 3
References:
1-Bianchimano P et al. 2023
2-Bolsega S et al. 2023
3-Yang L et al. 2023
Related and recommended products
For fluorometric detection, we recommend Trypsin Activity
Assay Kit (Fluorometric) Trypsin
Activity Assay Kit (Fluorometric)ab282916
This product is manufactured by BioVision, an Abcam company
and was previously called K771 Trypsin Activity Colorimetric Assay Kit.
K771-100 is the same size as the 100 test size of ab102531.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
