Lipid Peroxidation (MDA) Assay Kit
(Colorimetric/Fluorometric) (ab118970) provides a convenient tool for sensitive
detection of malondialdehyde (MDA).
How the assay works
In the lipid peroxidation assay protocol, the MDA in the sample reacts with
thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct can
be easily quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em
= 532/553 nm). This assay detects MDA levels as low as 1 nmol/well
colorimetrically and 0.1 nmol/well fluorometrically.
Lipid peroxidation assay protocol summary
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Add TBA solution to samples and standards, incubate at 95°C for 60 min,
cool in ice bath for 10 min
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Transfer to wells of microplate
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Analyze with microplate reader
For higher sensitivity, precipitate with n-butanol,
centrifuge, dry and resuspend pellet before analysis.
Related MDA assay products
For an alternative MDA assay, without the heating steps required in the TBARS
assay, try MDA assay Lipid
Peroxidation (MDA) Assay Kit (Colorimetric)ab233471.
Getting the best performance from ab118970
Technical Note: MDA is unlikely to be detectable in healthy urine but may be
elevated in the presence of infection or disease of the urinary tract.
How other researchers are using Lipid Peroxidation Assay
Kit ab118970
The MDA/TBARs assay kit has been used in publications in a variety of sample
types, including:
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Human: serum1, hippocampal primary cell extracts2,
A375 cultured cell lysates3, plasma and platelet samples4
- -
Mouse: neuronal cell lysates5, heart tissue extract6,
plasma7, cell extracts8
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Rat: hippocampal tissue extracts9, cardiomyocyte extracts of
cultured cells10, lung lysates11
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Pig: serum12
References: 1 - Shen J et al. 2018, 2 - Wang Q et al.
2019, 3 - Luo M et al. 2018, 4 - Mustafa AG et al. 2018, 5 - Murphy K et al.
2018, 6 - Guan F et al. 2019, 7 - Costa CRC et al. 2018, 8 - Eleftheriadis T et
al. 2019, 9 - Malekiyan et al. 2019, 10 - Zhou Z et al. 2018, 11 - Li L et al.
2018, 12 - Lee SE and Kang KS 2019
Related and recommended products
Also see the popular 4-HNE Assay Kit Lipid
Peroxidation (4-HNE) Assay Kitab238538 as an alternative marker of
lipid peroxidation and oxidative stress.
Iron Assay Kit Iron
Assay Kit (Colorimetric)ab83366 is often used in combination with
Lipid Peroxidation (MDA) Assay Kit ab118970 and GSH/ GSSG Assay Kit GSH+GSSG
/ GSH Assay Kit (Colorimetric)ab239709 in the study of ferroptosis.
Background information
Lipid peroxidation refers to the oxidative degradation of lipids. In this
process free radicals take electrons from the lipids (generally in cell
membranes), resulting in cell damage. Quantification of lipid peroxidation is
essential to assess oxidative stress. Lipid peroxidation forms reactive
aldehydes such as malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as
natural bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are
often used as markers of lipid peroxidation, and to assay for oxidative damage
/ oxidative stress.
The MDA assay is also refered to as a TBARS assay. The TBARS
assay (thiobarbituric acid reactive substance assay) is used to measure lipid
peroxidation in cell and tissue extracts, and biological fluids. The TBARS
assay detects the level of MDA (malondialdehyde), the major lipid oxidation
product, and also some minor related compounds. It is often considered a good
index of the level of oxidative stress in a biological sample.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously
called K739 Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit.
K739-100 is the same size as the 100 test size of ab118970.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
