abcam Glucose Uptake Assay Kit (Colorimetric)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C  

Appropriate long-term storage conditions

-20°C  

Storage information

-20°C  

How the assay works

Glucose Uptake Assay Kit (Colorimetric) is an assay that uses the glucose analog 2-deoxyglucose (2-DG) to detect and quantify glycose uptake in cells. 2-deoxyglucose (2-DG) is used in glucose uptake assay protocol because of its structural similarity to glucose. 2-DG is taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P cannot be further metabolized, and thus accumulates within cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In this assay, the 2-DG6P is oxidized to generate NADPH, the level of which can be determined by an enzymatic recycling amplification reaction.

Glucose uptake assay protocol summary:

- Prepare cells with suitable glucose starvation / uptake stimulation depending on experimental set-up

- Add 2-DG to cells and incubate for 20 mins at 37°C

- Wash cells with PBS to remove exogenous 2-DG

- Lyse cells with extraction buffer and repeated pipetting

- Freeze/thaw lysates and heat at 85°C for 40 min

- Cool on ice for 5 min

- Add neutralizing buffer, spin and transfer supernatant to new tubes

- Add supernatants and standards to wells

- Add reaction mix A and incubate for 1 hr at 37°C

- Add extraction buffer and heat to 90°C for 40 min

- Cool on ice for 5 min and add neutralizing buffer

- Add reaction mix B

- Analyze every 2-3 mins on microplate reader in kinetic mode at 37°C

How other researchers are using Glucose Uptake Assay ab136955

Glucose Uptake Assay Kit (Fluorometric) has been used in a variety of sample types including:

Human cervical epithelium HeLa and Human neuroblastoma SH-SY5Y 1

Human osteosarcoma cancer cell lines 143B and U2OS 2

Human breast cancer cell lines 3

References: 

1-Son S et al. 2023

2-Mei Z al. 2024

3-Peng Z et al. 2024

Related and recommended products

For fluorometric detection, we recommend Glucose Uptake Assay Kit (Fluorometric) Glucose Uptake Assay Kit (Fluorometric)ab136956

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye