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6-Phosphofructokinase Activity Assay kit ab155898 is simple,
robust, and rapid and can detect phosphofructokinase activity less than 1 mU. In the PFK activity assay protocol, PFK
(phosphofructokinase) converts fructose-6-phosphate and ATP to
fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme
mix is converted to AMP and NADH, which reduces a colorless probe to a colored
product with strong absorbance at 450 nm. PFK activity assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader over 20-60 min This product is manufactured by BioVision, an Abcam company
and was previously called K776 Phosphofructokinase (PFK) Activity Colorimetric
Assay Kit. K776-100 is the same size as the 100 test size of ab155898. Phosphofructokinase (PFK) is a key glycolytic enzyme and
plays a major regulatory role during glycolysis. This enzyme is present in
bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the
presence of ATP, PFK1 & PFK2 catalyzes the conversion of
fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate
respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle),
PFK-L (liver) and PFK-P (platelet). The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section. |
