Acetoacetate Assay Kit (Colorimetric) ab180875 adapts the
traditional qualitative assay using dipsticks, and sodium nitroferricyanide as
a chromophore, with a modification that provides a sensitive method to
quantitate endogenous levels of AcAc in human urine.
In this non-enzymatic assay, AcAc reacts with a substrate to
generate a colored product that can be measured at 550 nm. The reaction is
specific for AcAc and does not detect 3-β-hydroxybutyrate. The assay kit can
detect samples containing acetoacetate as low as 25 μM.
Acetoacetate assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader in kinetic mode for 10-15 min
This product is manufactured by BioVision, an Abcam company
and was previously called K650 Acetoacetate Colorimetric Assay Kit. K650-100 is
the same size as the 100 test size of ab180875.
Acetoacetate (AcAc), a β-ketoacid, is one of the three
ketone bodies and is formed via condensation of two molecules of acetyl-CoA in
liver mitochondria. AcAc can be enzymatically reduced to 3-β-hydroxybutyrate
(β-HB), or decarboxylated producing acetone ((CH3)2CO).
Ketone bodies [βHB: 78%; AcAc: 20% & (CH3)2CO: 2%]
are mainly used as an alternative energy source when glucose cannot be
delivered to the system. Excessive concentration of ketone bodies
(ketoacidosis) is observed in patients with Type I diabetes, severe starvation
or alcoholism. Traditionally, AcAc levels have been qualitatively detected
using dipsticks that use sodium nitroferricyanide as a chromophore.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
