Lipolysis Isolation and Quantification Assay Kit (Colorimetric/Fluorometric) (ab196987) is a simple assay to monitor
lipolysis using primary adipocytes. The kit provides all reagents necessary to isolate up to 5 grams of adipocytes from
mouse or rat adipose tissue and measure glycerol release after induction of lipolysis. It also includes a synthetic
catecholamine, isoproterenol, which stimulates the cAMP-mediated lipase activation pathway. The signal intensity is
directly proportional to the amount of glycerol present.
This product can detect from 20 pmol glycerol when using the fluorometric assay or from 200 pmol glycerol when
using the colorimetric assay.
This product is manufactured by BioVision, an Abcam company and was previously called K581 Lipolysis (Adipocyte)
Colorimetric/Fluorometric Assay Kit. K581-5 is the same size as the 5 g size of ab196987.
Lipolysis is the intracellular hydrolysis of triglycerides into glycerol and free fatty acids, which are then released into
the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown
adipose tissue. Deficiencies in lipolysis lead to increased intracellular lipid accumulation, resulting in abnormal
cellular physiology, hyperlipidemia, and insulin resistance. Lipolysis can be induced by hormones such as
catecholamine, which binds to β-adrenergic receptors leading to activation of adenylate cyclase, forming cAMP from
ATP. cAMP serves as a second messenger to activate hormone-sensitive lipase, which hydrolyzes the triglycerides.
This pathway is inhibited by insulin.
