Oxalate Assay kit (Colorimetric) (ab196990) is an easy-to-use, sensitive and high throughput adaptable kit. In this
assay, Oxalate reacts with oxalate converter and oxalate enzyme mix to form an intermediate that reacts in turn with
a highly specific probe to generate color that can be detected at OD=450 nm.
This product can detect Oxalate levels lower than 20 μM.
Oxalate assay protocol summary:
- add samples and standards to wells
- add oxalate convertor and incubate for 1 hr
- add reaction mix and incubate for 1 hr
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K663 Oxalate (Oxalic Acid)
Colorimetric Assay Kit. K663-100 is the same size as the 100 test size of ab196990.
Oxalate (C O ), in the form of Oxalic acid is present in many foods and beverages (e.g. spinach, tea etc.). It
accumulates in many plant tissues and play role in regulating pH, osmosis and calcium storage. In animals, oxalate is
either absorbed from dietary intake or produced from glycolate metabolism in liver. Under normal conditions, the
daily oxalate load can be excreted by kidney. However, hereditary defects can cause an increased level of oxalate,
which leads to hyperoxaluria and results in the formation of kidney stones. Therefore, measurement of oxalate level
is useful for the prevention, diagnosis and monitoring of kidney stones.
