Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Triose Phosphate Isomerase (TPI) Activity Assay Kit
(Colorimetric) (ab197001) provides a quick and easy way for monitoring the
activity of triose phosphate isomerase (TPI) in a variety of samples. In this
kit, triose phosphate isomerase (TPI) converts dihydroxyacetone phosphate into
glyceradehyde-3-phosphate, which reacts with the enzyme mix and developer to
form a colored product with strong absorbance at OD=450 nm.
The assay is simple, sensitive and high-throughput and can
detect TPI activity as low as 40 mU/mL.
TPI assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 20-40 min
This product is manufactured by BioVision, an Abcam company
and was previously called K670 Triose Phosphate Isomerase (TPI) Activity
Colorimetric Assay Kit. K670-100 is the same size as the 100 test size of
ab197001.
Triose Phosphate Isomerase (TPI, TIM, EC 5.3.1.1) is an
important enzyme for glycolysis. It catalyzes the reversible conversion of
dihydroxyacetone phosphate to glyceraldehyde-3-phosphate, thus maintaining the
equilibrium of these two triose phosphates. TPI connects glycolysis to pentose
phosphate pathway and lipid metabolism. It is a stable homodimer found in
almost all organisms.
In humans, TPI deficiency is a rare multisystem disorder and
leads to progressive neurological dysfunction, characterized by hemolytic
anemia, cardiomyopathy and progressive neuromuscular impairment.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
