Beta Lactamase Activity Assay Kit (Colorimetric) ab197008 is
a simple and sensitive assay that can detect and quantify the enzymatic
activity of beta lactamases, a large family of hydrolases.
The beta lactamase assay protocol is based on the hydrolysis
of the substrate Nitrocefin, a chromogenic cephalosporin, that results in the
generation of a colored product (detectable at OD=490 nm), which is directly
proportional to the amount of beta-lactamase activity.
This product can detect as low as 0.06 mU of beta-lactamase
activity from bacterial samples or from biological fluids infected with
beta-lactamase expressing bacteria.
Beta lacatmase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader in kinetic mode every 2-3 min for 30-60 min
This product is manufactured by BioVision, an Abcam company
and was previously called K803 Beta-Lactamase Activity Colorimetric Assay Kit.
K803-100 is the same size as the 100 test size of ab197008.
Beta Lactamases (β-Lactamases, βLs), are a large family of
hydrolases comprising more than 850 identified members expressed in
Gram-positive and Gram-negative bacteria. βLs can be classified according to
their substrate or inhibitor specificity. These enzymes are capable of
hydrolyzing four atom rings known as β-lactams.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
