How the assay works
In Tyrosinase Inhibitor Assay Kit (Colorimetric) protocol,
tyrosinase catalyzes the oxidation of tyrosine, producing a chromophore that
can be detected at 510 nm. In the presence of kojic acid, a reversible
inhibitor of tyrosinase, the rate of oxidation of the substrate is decreased.
The absorbance values are used to calculate the tyrosinase activity of the
samples
Tyrosinase Inhibitor Assay Kit (Colorimetric)protocol
summary:
- Screening compound preparation
- Preparation of controls
- Enzyme and substrate solution preparation
- Add enzyme solution and incubate at 25°C for 10 minutes
- Add substrate solution
- Measure absorbance (OD 510 nm) in a kinetic mode for 30-60 minutes at 25°C
How other researchers are using
Tyrosinase Inhibitor Assay Kit (Colorimetric) has been used
in a variety of sample type including:
- Escherichia coli Salmonella enterica and Pseudomonas aeruginosa1
Highly ripe red guava extracts2
Staphylococcus aureus3
References:
1-Vilas-Boas A et al. 2023
2-Bao S et al. 2023
3-Oliveira A et al. 2022
Related and recommended products
For quantify Tyrosinase Activity, we recommend Tyrosinase
Activity Assay Kit (Colorimetric) Tyrosinase
Activity Assay Kit (Colorimetric)ab252899
This product is manufactured by BioVision, an Abcam company
and was previously called K575 Tyrosinase Inhibitor Screening Kit
(Colorimetric). K575-100 is the same size as the 100 test size of ab204715.
Tyrosinase or polyphenol oxidase (EC 1.14.18.1), is an
oxidoreductase that participates in the biosynthesis of melanin, a ubiquitous
biological pigment found in hair, eyes, skin, etc. Inhibition of tyrosinase has
been a long-time target in the skin health research, cosmetics and agricultural
industries because of its role in browning reactions in skin pigmentation and
during fruit harvesting and handling. Skin whitening and bleaching products
utilize natural or synthetic tyrosinase inhibitors in order to lighten the skin
color. Polyphenols, benzaldehyde derivatives, long-chain lipids, steroids, and
natural compounds have been used as tyrosinase inhibitors.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
