abcam Acetate Assay Kit (Colorimetric)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

How the assay works

In Acetate Assay Kit (Colorimetric) protocol, free acetate is converted to an intermediate in the presence of Acetate Enzyme Mix and Acetate Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD=450 nm.

Acetate assay protocol summary:

- Add samples and standards to wells

- Add reaction mix

- Incubate for 40 min

- Analyze with microplate reader

How other researchers are using

Acetate Assay Kit (Colorimetric) has been used in a variety of sample type including:

- E. coli and Enterobacter supernatant 1

- Mouse spinal cord and serum samples 2

- Human Esophageal squamous cell carcinoma cell lines 3

References: 

1-Estrela S al. 2022

2-Jin S et al. 2021

3-Odera J et al. 2020

This product is manufactured by BioVision, an Abcam company and was previously called K658 Acetate Colorimetric Assay Kit. K658-100 is the same size as the 100 test size of ab204719.

Acetate is an important component for biosynthesis. In living organisms, acetylation/deacetylation of proteins is critical for posttranslational regulation of their functions. When bound to CoA, Acetyl-CoA is central to metabolism of carbohydrates and fats. It is also involved in the synthesis of biogenic compounds such as neurotransmitter acetylcholine.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye