Nitric Oxide Synthase Activity Assay Kit (Colorimetric) (ab211083) provides an accurate and convenient method to
assay nitric oxide synthase (NOS) activity in a variety of samples. In this assay, nitric oxide(NO) generated by NOS
undergoes a series of reactions and reacts with Griess Reagent 1 and 2 to generate a colored product with a strong
absorbance at OD 540 nm. The assay is simple, sensitive and high-throughput adaptable and can detect as low as 5
μU of NOS activity.
This product is manufactured by BioVision, an Abcam company and was previously called K205 Nitric Oxide Synthase
(NOS) Activity Assay Kit (Colorimetric). K205-100 is the same size as the 100 test size of ab211083.
Nitric oxide synthases (EC 1.14.13.39) (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO)
from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune
response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme,
NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate
NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS).
nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and
information storage. eNOS produces NO in blood vessels and is involved in regulation of vascular function. In
contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts
of NO as a part of body's defense mechanism.
