Adenosine Deaminase (ADA) Activity Assay Kit (Colorimetric)
(Adenosine
Deaminase (ADA) Activity Assay Kit (Fluorometric)ab204695) is an assay
where inosine formed from the breakdown of adenosine is detected via a
multi-step reaction, resulting in the formation of an intermediate that reacts
with the ADA convertor and developer to generate uric acid that can be easily
quantified at OD293 nm.
The kit measures total activity of Adenosine Deaminase with
limit of quantification of 1 mU recombinant Adenosine Deaminase.
This product is manufactured by BioVision, an Abcam company
and was previously called K321 Adenosine Deaminase Activity Assay Kit
(Colorimetric). K321-100 is the same size as the 100 test size of ab211093.
Adenosine Deaminase (ADA, EC 3.5.4.4) is an enzyme that
catalyses the conversion of adenosine and 2'-deoxyadenosine to inosine and
2'-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues
and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in
most cells in the body, particularly in lymphocytes and macrophages. It is
present in the cytosol, nucleus and has been found associated with DPP4/CD26 in
the cell membrane. ADA2 was first found in the spleen but is predominantly
found in the plasma and serum. Increased serum ADA levels are found in certain
infectious diseases such as tuberculosis and various liver diseases such as
acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a
few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
