How the assay works
In Succinate Dehydrogenase Activity Assay Kit
(Colorimetric), Succinate Dehydrogenase converts succinate to fumarate, and
transfers the electron to an artificial electron acceptor (Probe), which
changes the color from blue to a colorless product (depending upon the sample
enzymatic activity).
Succinate Dehydrogenase Activity Assay Kit (Colorimetric)
protocol summary:
- Rapidly homogenize sample tissue / cells in assay buffer
- Centrifuge sample and transfer supernatant to fresh tube
- Add sample to plate well
- Add reaction buffer and mix well
- Immediately measure absorbance (600 nm) in kinetic mode for 10 –30 minutes at
25°CThe kit provides enough reagents for 100 assays.
How other researchers are using
Succinate Dehydrogenase Activity Assay Kit (Colorimetric)
has been used in a variety of sample type including:
- Mouse liver mitochondria 1
- Rat brain tissues 2
- HepG2 and MCF-7 cells 3
References: 1-Rome F al. 2022
2 Almohaimeed H et al. 2022
3- Liu X et al. 2021
This product is manufactured by BioVision, an Abcam company
and was previously called K660 Succinate Dehydrogenase Activity Colorimetric
Assay Kit. K660-100 is the same size as the 100 test size of ab228560.
Succinate Dehydrogenase (SDH) (EC 1.3.5.1) or
succinate-coenzyme Q reductase (SQR) or respiratory complex II is an enzyme
complex, which is bound to the inner mitochondrial membrane. SDH participates
in both the citric acid cycle and electron transport chain. In mammals and many
bacteria, SDH consists of 2 hydrophilic subunits, SDHA (flavoprotein) and SDHB
(iron-sulfur protein) and 2 hydrophobic membrane anchor subunits: SDHC and
SDHD. SDH oxidizes succinate to fumarate and transfers the electrons to ubiquinone.
SDH deficiency in humans leads to a variety of phenotypes including Leigh
syndrome, a neurometabolic disorder, tumor formation, and myopathy. Recent
studies show that SDH can prevent the generation of ROS (reactive oxygen
species); therefore, measurement of succinate dehydrogenase activity has wide
applications.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
