How the assay works
In Pyrophosphate Assay Kit (Colorimetric / Fluorometric)assay protocol, PPi produced during biotic processes is detected through a series of reactions which utilize a proprietary enzyme mix and probe, generating a stable product that can be quantified by either colorimetric or fluorometric readout. Generated fluorescence (Ex/Em = 535/587 nm) or color (OD: 570 nm) intensities are directly proportional to the concentrations of pyrophosphate, enabling precise measurements. Monomeric inorganic phosphate (Pi) does not interfere with the assay.
Pyrophosphate Assay Kit (Colorimetric / Fluorometric)protocol summary:
- Prepare samples.
- Prepare standard curve.
- Prepare reaction mix.
- Add samples, controls and standards to wells and incubate plate protected from light.
- Measure absorbance (570 nm) or fluorescence EX/Em = 535/587 nm).
Related and recommended products
See an alternative Pyrophosphate Assay Kit:
Pyrophosphate Assay Kit (Fluorometric) Pyrophosphate Assay Kit (Fluorometric)ab112155
This product is manufactured by BioVision, an Abcam company and was previously called K568 Pyrophosphate (PPi) Assay Kit (Fluorometric/Colorimetric). K568-100 is the same size as the 100 test size of ab234040.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
