Bile Acid Assay Kit (Colorimetric) ab239702 provides a
simple, sensitive, and high-throughput suitable approach to quantify the
concentration of total bile acid in biological fluids.
The bile acid assay is based on an enzymatic cycling method
in the presence of NADH and a chromophore. The reduction of the chromophore
produces a stable colorimetric product the absorbance of which can be followed
kinetically at 405 nm on a multi-well spectrophotometer. This absorbance is
directly proportional to the amount of total bile acids in the sample.
Other metabolites found in biofluids do not interfere with
the assay.
The assay can detect as little as 1 μM of Bile Acids in a
variety of samples.
Bile acid assay protocol summary:
- add samples and standards to wells
- add probe mix and incubate at 37°C for 10 min
- add reaction mix
- measure absorbance at 405 nm for 60 min at 37°C in a kinetic mode
This product is manufactured by BioVision, an Abcam company
and was previously called K209 Total Bile Acids (TBA) Assay Kit (Colorimetric).
K209-100 is the same size as the 100 test size of ab239702.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
