abcam Alpha Galactosidase Activity Assay Kit

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Alpha Galactosidase Activity Assay Kit ab239716 provides a simple, rapid way to monitor total alpha galactosidase (alpha-Gal) activity in a wide variety of biological samples.

How the assay works

In this kit, α-Gal cleaves a synthetic specific substrate releasing a fluorophore, which can be easily quantified (Ex/Em= 360/445 nm). The assay is specific, sensitive and can detect as low as 0.1 μU of α-Galactosidase activity. This kit does not detect beta galactosidase activity.

Alpha Galactosidase assay methods

There are two methods used for alpha galactosidase assays:

The most common method uses 4-Methylumbelliferyl-alpha-D-galactopyranoside, as an alpha galactosidase substrate. Alpha galactosidase cleaves the substrate releasing fluorescent Methylumbelliferone. This is the method used in alpha galactosidase assay kit ab239716.

The alternative, less-popular method uses the substrate 4-nitrophenyl-α D galacto-pyranoside. Similarly, alpha galactosidase cleaves the substrate releasing pNA, which can be detected by absorbance.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye