abcam Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (ab273328) provides a quick and easy method for monitoring PHGDH activity in a variety of samples. In this kit, Phosphoglycerate Dehydrogenase converts 3-phosphoglycerate and NAD into 3-phosphohydroxyglerate and NADH respectively. The oxidation of NADH reduces a probe generating a strong, stable absorbance signal (OD: 450 nm).

The assay is simple, sensitive, high–throughput adaptable and can detect Phosphoglycerate Dehydrogenase activity less than 0.1 mU per sample.

This product is manufactured by BioVision, an Abcam company and was previously called K569 Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric). K569-100 is the same size as the 100 test size of ab273328.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.

It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. 

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye