The Mitochondrial Complex I assay kit ab287847 is a fast and reliable method to determine the activity of complex I in isolated mitochondria. It is useful for respiration studies in isolated mitochondria and may be used to study effects of toxicants, drugs and other environmental conditions on mitochondrial complex I activity. This kit uses decylubiquinone, an analog of ubiquinone, as an electron acceptor that gets converted to decylubiquinol through the catalytic activity of Complex I. The Complex I dye that absorbs light at 600 nm in its oxidized form is used as a terminal electron acceptor that accepts electrons from decylubiquinol. Complex I activity is determined colorimetrically by recording the change in absorbance of reduced Complex I dye at 600 nm. Specific Complex I activity is obtained by subtracting the activity in presence of Complex I inhibitor rotenone from total activity. This kit can detect as low as 0.1 mU / well and is linear up to 7 mU / well.
This product is manufactured by BioVision, an Abcam company and was previously called K968 Mitochondrial Complex I Activity Colorimetric Assay Kit. K968-100 is the same size as the 96 test size of ab287847.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
