abcam L-Lactate Assay Kit (Colorimetric/Fluorometric)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

L-Lactate Assay Kit (Colorimetric/Fluorometric) ab65330 provides a convenient means for detecting L(+)-Lactate in biological samples such as blood, cells, culture mediums, fermentation mediums, etc. There is no need for pretreatment or purification of samples.

How the assay works

In the lactate assay protocol, lactate specifically reacts with an enzyme mix to generate a product, which interacts with a lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm).

Lactate assay protocol summary

• - Add samples (deproteinized) and standards to wells.
• - Add reaction mix and incubate for 30 min at room temp.
• - Analyze with microplate reader.

Related Lactate assay products

Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range:

• - This kit L-Lactate assay ab65330: colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM.
• - Our most popular L-Lactate assay kit L-Lactate Assay Kit (Colorimetric)ab65331: colorimetric 450nm, range 0.02 mM - 10 mM.
• - L-Lactate assay kit L-Lactate Assay Kit (Fluorometric, High Sensitivity)ab169557: fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM.

L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate.

We also provide D-Lactate assay kit D-Lactate Assay Kit (Colorimetric)ab83429.

Lactate assay methods

There are two lactate assay methods established in biological research:

• a) The most commonly used method, where lactate dehydrogenase processes lactate in the presence of NAD+ to produce pyruvate and NADH. NADH then reduces a probe to form a colorimetric or fluorometric readout. This method, with some improvements, is used in L-Lactate Assay Kit (Colorimetric)ab65331 and in L-Lactate Assay Kit (Fluorometric, High Sensitivity)ab169557.
• b) The alternative method, where lactate oxidase processes lactate to produce pyruvate and hydrogen peroxide. This is followed by oxidation of a substrate by a peroxidase using the hydrogen peroxide to produce a colorimetric or fluorometric readout. This method is used in ab65330.

Other notes

This product is manufactured by BioVision, an Abcam company and was previously called K607 Lactate Colorimetric/Fluorometric Assay Kit. K607-100 is the same size as the 100 test size of ab65330.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.

It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

The quality of HiYield™ Plasmid Mini Kit is test on a lot-to-lot basis. The kits are tested by isolation of plasmid DNA from 3ml culture of E.coli DH5 transformed with the plasmid pUC19(A600>2units/ml). More than 20μg of plasmid DNA was quantified with spectrophotometer.

•  QPS-101T
  • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
  • 50x ROX reference dye    50 µl × 1
•  
• QPS-101
  • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
  • 50x ROX reference dye    250 µl × 1
•  
• QPS-101X5
  • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
  • 50x ROX reference dye    250 µl × 5

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye

• Hot Start
• Probe
• 2x Reaction mix
• 20µl total reaction volume
• Store at -20oC
• 50x ROX reference dye