L-Lactate Assay Kit (Colorimetric) (ab65331) uses an assay
protocol where lactate is oxidized by lactate dehydrogenase to generate a
product which interacts with a probe to produce a color (λmax = 450 nm).
The kit detects L(+)-Lactate in biological samples such as
serum or plasma, cells, tissues, cell culture and fermentation media.
Lactate assay protocol summary
- -
Add samples and standards to wells.
- -
Add reaction mix and incubate for 30 min at room temp.
- -
Analyze with microplate reader.
Related Lactate assay products
Alternative L-Lactate assay kits offer different readout modes/wavelengths and
sensitivity/range:
L(+)-Lactate is the major stereo-isomer of lactate formed in
human intermediary metabolism and is present in blood. D(-)-Lactate is also
present but only at about 1-5% of the concentration of L(+)-Lactate.
We also provide D-Lactate assay kit D-Lactate
Assay Kit (Colorimetric)ab83429.
How other researchers are using L-Lactate Assay Kit
ab65331
This Lactate assay kit has been used in publications in a variety of sample
types, including:
- -
Human: THP-1 cell lysates1, MDA-MB-231 and HepG2 cell culture
lysates2, cell culture supernatant (HepG2, A549, Huh7, PC3,
LN229, HeLa)3, brain tissue4
- -
Mouse: brown adipose tissue lysate5, thymic lymphoma tissue6,
cell culture supernatant7, T cell primary cell culture
supernatants8, serum9, serum and muscle10
- -
Bovine: cumulus cell culture supernatant11
References: 1 - Tran UT and Kitami T 2019; 2 - Cui J et
al 2019; 3 - Rodriguez ML et al 2018, Chen Y et al 2018, Zhang D et al 2018,
Caino MC et al 2017, Birkenmeier K et al 2015; 4 - Sullivan RC et al 2019; 5 -
Jeong JH et al 2018; 6 - Vara-Ciruelos D et al 2019; 7 - Fiorenzano et al 2016;
8 - Menk AV et al 2018; 9 - Deng W et al 2019, Guglielmetti C et al 2017, Kang
R et al 2016; 10 - Kim HY et al 2016; 11 - Sinha et al 2017.
Lactate assay methods
There are two lactate assay methods established in biological research:
- a)
The most commonly used method, where lactate dehydrogenase processes
lactate in the presence of NAD+ to produce pyruvate and NADH. NADH then
reduces a probe to form a colorimetric or fluorometric readout. This
method, with some improvements, is used in ab65331 and in L-Lactate
Assay Kit (Fluorometric, High Sensitivity)ab169557.
- b)
The alternative method, where lactate oxidase processes lactate to produce
pyruvate and hydrogen peroxide. This is followed by oxidation of a
substrate by a peroxidase using the hydrogen peroxide to produce a
colorimetric or fluorometric readout. This method is used in L-Lactate
Assay Kit (Colorimetric/Fluorometric)ab65330.
Other notes
This product was previously called K627 Biovision Lactate Colorimetric Assay
Kit II. Abcam acquired BioVision in 2021.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
