Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339)
provides an accurate, convenient measure of creatine in a variety of biological
samples.
In the creatine assay protocol, creatine is enzymatically
converted to sarcosine which is then specifically oxidized to generate a
product that converts a colorless probe to an intensely red color (λmax = 570
nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore
easily detected by either colorimetric or fluorometric methods. Detection range
0.001–10 mM Creatine.
Creatine assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 60 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company
and was previously called K635 Creatine Colorimetric/Fluorometric Assay Kit.
K635-100 is the same size as the 100 test size of ab65339.
Creatine is an endogenous compound whose function is to
maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine
kinase. Creatine supplementation has been used in the treatment of muscular,
neurological and neurodegenerative diseases, as well as a sport performance
enhancer. Detection of creatine level has wide applications in research and
development.
Abcam has not and does not intend to apply for the REACH
Authorisation of customers' uses of products that contain European
Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application
of REACH Authorisation, and any other relevant authorisations, for their
intended uses.
