Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
Superoxide Dismutase Activity Assay Kit (Colorimetric)
ab65354 is a simple and rapid assay for superoxide dismutase (SOD) activity.
In the SOD assay protocol:
- superoxide anions are produced by the action of xanthine oxidase
- SOD catalyzes the dismutation of the superoxide anion into hydrogen peroxide
and O2
- superoxide anions act on WST-1 to produce a water-soluble formazan dye which
can be detected by the increase in absorbance at 450 nm
The greater the activity of SOD in the sample, the less formazan dye is
produced.
Superoxide dismutase assay protocol summary:
- add samples to wells
- add WST-1 working solution and enzyme working solution and incubate for 20
min at 37°C
- analyze with microplate reader
Superoxide dismutase (SOD) is one of the most important
antioxidative enzymes. It catalyzes the dismutation of the superoxide anion
into hydrogen peroxide and molecular oxygen. **Related products** Review the to
learn about more assays for oxidative stress.
The Safety Datasheet for this product has been updated for
certain countries. Please check the current version in the Support and
downloads section.
